名稱 | NGF/Canine TRKA Effector Reporter Cell |
型號 | CBP74230 |
報(bào)價(jià) | |
特點(diǎn) | NGF/Canine TRKA Effector Reporter Cell |
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藥靶細(xì)胞株 > 腫瘤免疫細(xì)胞株 > CBP74230NGF/Canine TRKA Effector Reporter Cell
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NGF/Canine TRKA Effector Reporter Cell
I. Background | |
NGF 是神經(jīng)營養(yǎng)因子(NT)家族的成員,該家族還包括腦源性神經(jīng)營養(yǎng)因子 (BDNF)、神經(jīng)營養(yǎng)因子 3(NT-3)和神經(jīng)營養(yǎng)因子 4/5(NT-4/5)。神經(jīng)營養(yǎng)因子是發(fā) 育、維持和存活以及記憶形成和儲存的關(guān)鍵調(diào)節(jié)因子。NGF 在成年基底前腦能神經(jīng)元 (BFCN )的刺激、存活和表型維持中起著關(guān)鍵作用。它在皮質(zhì)和海馬體中合成,并逆行 輸送到 BFCN 細(xì)胞體,觸發(fā)導(dǎo)致維持正常細(xì)胞功能和形態(tài)的過程。NGF 可以上調(diào)幾種能標(biāo)志物,即 ChAT 活性、基因表達(dá)和蛋白質(zhì)水平、乙酰合成和釋放以及囊泡乙酰轉(zhuǎn)運(yùn)蛋白(VAChT)的表達(dá)。 | |
II. Description | |
NGF/Canine TRKA Effector Reporter Cell 報(bào)告基因藥靶模型很好的模擬了體內(nèi) NGF/Canine TRKA 的信號轉(zhuǎn)導(dǎo)過程,原理見下圖所示。
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III.Introduction | |
Expressed gene: | NGF/Canine TRKA |
Stability: | 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
Synonym(s): | nerve growth factor (beta polypeptide), NGFB |
Freeze Medium: | 90% FBS+10%DMSO |
Culture Medium: | DMEM+10%FBS+2 μg/ml puromycin+200 μg/ml hygromycin |
Mycoplasma Testing: | Negative |
Storage: | Liquid nitrogen |
Application(s): | Functional(Report Gene) Assay |
IV. Representative Data |
Figure 2.Dose Response of Recombinant Human β-NGF Protein in NGF/Canine TRKA Effector Reporter Cell (C19).
Figure 3. Inhibition of NGF-induced Reporter Activity by Canine NGF Neutralization Ab in NGF/Canine TRKA Effector Reporter Cel1 (C19).