名稱 | hMET/LL/2 |
型號(hào) | CBPG0146 |
報(bào)價(jià) | |
特點(diǎn) | hMET/LL/2 |
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聯(lián)系人:蔣經(jīng)理
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電話:4008750250
號(hào)碼:
手機(jī):18066071954
地址:南京市棲霞區(qū)緯地路9號(hào)
Email: zhangxiangwen@cobioer.com
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hMET/LL/2
I. Background | |||
C-Met 屬于受體激酶家族,是一種異二聚體,由高度糖基化的細(xì)胞外 50 kDa α 鏈和跨膜 140 kDa β鏈組成,通過二硫鍵連接在一起。作為一種天然的 HGF 受體,它表達(dá) 于各種上皮細(xì)胞的表面,并促進(jìn)腫瘤的發(fā)生。與 HGF 結(jié)合后,兩個(gè)亞基二聚化,導(dǎo)致兩個(gè) 殘基(Tyr1234 和 Tyr1235 )自磷酸化。殘基的自磷酸化導(dǎo)致一個(gè)激活的多功 能停靠位點(diǎn),該位點(diǎn)由 SH2 結(jié)構(gòu)域、PTB 結(jié)構(gòu)域和 Met 結(jié)合結(jié)構(gòu)域 (MDB) 組成,該結(jié) 構(gòu)域募集接頭分子。 | |||
II. Introduction | |||
Host Cell: | LL/2 | ||
Expressed gene: | hMET | ||
Stability: | 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) | ||
Freeze Medium: | 90% FBS+10% DMSO | ||
Culture Medium: | DMEM+10%FBS+10 μg/ml blasticidin | ||
Mycoplasma Testing: | Negative | ||
Storage: | Liquid nitrogen | ||
III. Description of Host Cell Line | |||
Organism: | Mus musculus, mouse | ||
Tissue: | lung | ||
Morphology: | rounded - loosely attached or floating | ||
Growth Properties: | mixed, adherent and suspension |
IV. Representative Data | |||
Figure 1. Recombinant hMET/LL/2 stably expressing hMET. | |||