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藥靶細胞株 > 腫瘤免疫細胞株 > CBP74034CD40/CHO

CD40/CHO
名稱 CD40/CHO
型號 CBP74034
報價
特點 CD40/CHO，凍存條件：90% FBS+10% DMSO
  • 詳細內(nèi)容
CBP74034
I. Background
CD40, a TNF receptor superfamily member, was initially identified on B lymphocytes. However, other cell types such as monocytes, basophils, dendritic cells, endothelial cells, and epithelial cells have been found to express CD40. High levels of CD40 have also been detected in numerous human cancers, including HCT116, a colon cancer epithelial cell line. Interaction with CD40 ligand (CD40L, CD154) on CD4+ T helper lymphocytes triggers the expression of intercellular adhesion molecule (ICAM) and other pro-inflammatory cytokines. CD40:CD40L signaling simultaneously increases activation of antigen-specific T cells. CD40 also activates NF-κB-dependent signaling in response to lipopolysaccharide (LPS) found on Gram negative bacterial pathogens. Agonistic CD40 monoclonal antibodies have been shown to activate antigen presenting cells (APC), promote anti-tumor T-cell responses, and to foster cytotoxic myeloid cells, suggesting a potential mechanism to control cancer in the absence of T-cell immunity.

II. Description

Recombinant CHO cell line expressing full length human CD40 (Tumor necrosis factor receptor superfamily member 5; TNFRSF5), Genbank Accession No. NP_001241.1. 


III. Introduction
Host Cell:CHO
Expressed gene:CD40
Stability:32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.)
Freeze Medium:90% FBS+10% DMSO
Culture Medium:

F12k+10%FBS+5ug/ml puromycin

Application(s):Binding Assay,FACS
Storage:Liquid nitrogen

IV. Description of Host Cell Line
Organism:Cricetulus griseus, hamster, Chinese
Tissue:Ovary
Disease: Hamster Chinese ovary
Morphology: Epitheloid cell
Growth Properties: Adherent

Ⅴ. Representative Data
COBIOER

Figure 1. Recombinant CHO cell line expressing full length human CD40.






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