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藥靶細(xì)胞株 > kinase激酶細(xì)胞株 > CBP73236c-Kit D820Y/BaF3

c-Kit D820Y/BaF3
名稱 c-Kit D820Y/BaF3
型號 CBP73236
報(bào)價(jià)
特點(diǎn) c-Kit D820Y/BaF3
  • 詳細(xì)內(nèi)容

CBP73236

I. Introduction

Cell Line Name:

c-Kit D820Y/BaF3

Host Cell:

Ba/F3

Stability:16 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.)

Application:

Anti-proliferation assay and PD assay

Freeze Medium:

90% FBS+10% DMSO

Complete Culture Medium:

RPMI-1640+10%FBS

Mycoplasma Status:

Negative

 
II.Background

KIT D820Y lies within the protein kinase domain of the Kit protein (UniProt.org). D820Yresuts in constitutive phosphorylation of Kit and is transforming in cell culture (PMID: 19035443, PMID: 11984533), and has also been identified as a secondary mutation associated with imatinib-resistance (PMID: 18488160).

 
III. Representative Data

1. WB of c-Kit D820Y/BaF3

2.Sanger of c-Kit D820Y/BaF3

3. Anti-proliferation assay

Figure 3. CTG Proliferation Assay of BaF3 c-KIT D820Y (C1).

 

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