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藥靶細(xì)胞株 > kinase激酶細(xì)胞株 > CBP73314c-Kit V560Del/BaF3

c-Kit V560Del/BaF3
名稱 c-Kit V560Del/BaF3
型號(hào) CBP73314
報(bào)價(jià)
特點(diǎn) c-Kit V560Del/BaF3
  • 詳細(xì)內(nèi)容

CBP73314

I. Introduction

Cell Line Name:

c-Kit V560Del/BaF3

Host Cell:

BA/F3

Stability:16 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.)

Application:

Anti-proliferation assay and PD assay

Freeze Medium:

90% FBS+10% DMSO

Complete Culture Medium:

RPMI-1640+10%FBS

Mycoplasma Status:

Negative

 
II.Background
KIT V560del (also reported as V559del) results in the deletion of an amino acid in the juxtamembrane domain (exon 11) of the Kit protein at amino acid 560 (PMID: 12879016). V560del confers a gain of function to Kit, resulting in constitutive, ligand independent phosphorylation of Kit and activation of Akt in cell culture (PMID: 15236194, PMID: 22282465), and transformation of cultured cells (PMID: 19861435).
 
III. Representative Data

1. WB of c-Kit V560Del/BaF3

2. Sanger of c-Kit V560Del/BaF3

3. Anti-proliferation assay

Figure 3. CTG Proliferation Assay of BaF3 c-KIT V560del (C7).

 

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