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藥靶細(xì)胞株 > kinase激酶細(xì)胞株 > CBP73333PDGFRA D842V-V658A/BaF3 kinase細(xì)胞株

PDGFRA D842V-V658A/BaF3 kinase細(xì)胞株
名稱 PDGFRA D842V-V658A/BaF3 kinase細(xì)胞株
型號(hào) CBP73333
報(bào)價(jià)
特點(diǎn) PDGFRA D842V-V658A/BaF3
  • 詳細(xì)內(nèi)容
CBP73333
I. Introduction

Cell Line Name:

PDGFRA D842V-V658A/BaF3

Host Cell:

Ba/F3

Stability:16 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.)

Freeze Medium:

90% FBS+10% DMSO

Complete Culture Medium:

RPMI-1640+10%FBS

Mycoplasma Status:

Negative

II.Background

PDGFRA D842V lies within the protein kinase domain of the Pdgfra protein (UniProt.org). D842V results in ligand-independent phosphorylation of Pdgfra, activation of downstream signaling, and is transforming in culture (PMID: 22745105, PMID: 23752188, PMID: 29533785, PMID: 30253204) and confers resistance to PDGFRA inhibitors (PMID: 30389923, PMID: 18955458).

III. Representative Data

1.Sanger of PDGFRA  D842V-V658A/BaF3


Figure 1. PDGFRA D842V-V658A/BaF3  V658A

Figure 2. PDGFRA D842V-V658A/BaF3 D842V

2. Anti-proliferation assay

CBP73333-3.jpg


Figure 3. CTG Proliferation Assay of BaF3 PDGFRA D842V/V658A Cells (C1).




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